Kinetic Studies of β-Galactosidase Induction
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چکیده
منابع مشابه
Kinetic Analysis of Guanidine Hydrochloride Inactivation of β-Galactosidase in the Presence of Galactose
Inactivation of purified β-Galactosidase was done with GdnHCl in the absence and presence of varying [galactose] at 50°C and at pH 4.5. Lineweaver-Burk plots of initial velocity data, in the presence and absence of guanidine hydrochloride (GdnHCl) and galactose, were used to determine the relevant K(m) and V(max) values, with p-nitrophenyl β-D-galactopyranoside (pNPG) as substrate, S. Plots of ...
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es, such as α-mannosidases (MAN) and β-galactosidases (GAL), are found in different glycoside hydrolase sequencebased families. Considerable research has proved plays the role of MAN, which play a key role in the modification and diversification of hybrid N-glycans, processes with strong cellular links to cancer. Therefore the study aim was to investigate the activities of MAN and GAL in larynx...
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The effects of lactose, galactose, glucose, and glucose combined with galactose on the induction of β–galactosidase by IPTG in Escherichia coli strain VC10, growing in a glycerol media, were compared against a control of glycerol plus IPTG. After inducing β– galactosidase production with the IPTG, the cultures were altered with the addition of one of the above carbohydrates or combinations. Thi...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 1961
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(61)86913-0